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| Cardoso,Jean C; Ono,Elizabeth O. |
| One of the most important applications of plant tissue culture is mass propagation of ornamental plants. This experiment evaluated the effect of different concentrations of NH4NO3 and KNO3 and BAP on the in vitro growth of orchid hybrid Brassocattleya 'Pastoral'. Seedlings of this orchid hybrid were used as explants and cultivated in medium with mineral salts and vitamins from the MS medium (Murashige & Skoog, 1962), with the macronutrients P, Ca and Mg reduced by half, and with an addition of 25 g L-1 of sucrose, 0.1 g L-1 of myo-inositol and 1.5 g L-1 of activated charcoal. Agar-agar was added (6.5 g L-1) and the pH was adjusted to 5.8. As treatments, four concentrations of the NH4NO3 and KNO3 (2x; 1x; ½ and ¼ MS medium) and three concentrations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tissue culture; Macronutrients; Cytokinins; Multiplication; Growth. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000300017 |
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| Markovic,Marija; Grbic,Mihailo; Djukic,Matilda. |
| The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and half-strength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newly-developed shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pineleaf pink; In vitro culture; BAP; NAA; Multiplication. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100403 |
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| Pêgo, Rogério Gomes; Universidade Federal de Viçosa; Paiva, Patrícia Duarte de Oliveira; Universidade Federal de Lavras; Paiva, Renato; Universidade Federal de Lavras. |
| The research aimed to establish a protocol of micropropagation of Syngonanthus elegans. Seed germination in WPM media containing 0%, 25%, 50%, 75% and 100% salt concentrations was evaluated. Establishment of plantlets in WPM and MS media, with 50% or 100% salt concentrations, was studied. The addition of 0, 5, 10, 15, 20, 25 and 30 g L-1of sucrose in WPM media was evaluated. The effects of TDZ (0.0; 0.5; 1.0; 2.0 and 4.0 mg L-1) and ANA (0.0; 0.5 and 1.0 mg L-1) in all possible combinations were investigated. To test acclimatization, sand, PlantmaxTM or vermiculite substrates were evaluated by direct acclimatization and pre-acclimatization. The germination of S. elegans seeds was not influenced by salt concentration in the culture media, but the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.01.00.00-9 floriculture; In vitro cultivation; Multiplication; Acclimatization Agronomia. |
| Ano: 2014 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/17946 |
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| CORREIA, D.; BERNARDO, J. C.. |
| Echinocactus grusonii, conhecido como ?Cactus cadeira-desogra?, é uma espécie endêmica da região central do México que se encontra ameaçada de extinção. Possui ocorrência em locais semidesérticos, apresenta potencial ornamental e os frutos são utilizados na alimentação humana. A reprodução ocorre exclusivamente por ementes, como na maioria dos cactos globulares. Assim, a micropropagação torna-se uma ferramenta importante na produção de mudas e conservação da espécie. O objetivo do estudo foi avaliar a germinação, o crescimento de plântulas in vitro e a micropropagação de E. grusonii. Echinocactus grusonii, known as ?mother-in-law cactus chair?, is an endemic species in central Mexico and endangered. Occurs in semidesert locations. It has a high ornamental... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Echinocactus grusonii; Cacto cadeira-de-sogra; Multiplicação; Aclimatização; Golden ball cactus; Multiplication; Acclimatization. |
| Ano: 2020 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1125421 |
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